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This study analyzes urban youths’ career decision-making in a career and technical education program that provided work-based learning experiences and a pathway linking high school, community college, and work in biotechnology laboratories. The program provided work experiences not generally available to adolescents, and enabled students underrepresented in the sciences to experience and envision a scientific career. Case studies reveal tensions in students’ career decision-making as they gained knowledge/skills and kept career and educational options open; students saw increasing options for their future and the limits of program experiences. Students should be supported to take on active roles and to shape educational and career programs to fit their unique goals.